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High-Performance Liquid Chromatographic Analysis of Cytokinins Using Isatoic Anhydride as a Fluorescent Derivatizing Reagent

✍ Scribed by Sonoki, S.; Sono, Y.; Hisamatsu, S.; Sugiyama, T.

Book ID
127022862
Publisher
Informa UK (Taylor & Francis)
Year
1993
Tongue
English
Weight
406 KB
Volume
16
Category
Article
ISSN
0148-3919

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A method which uses 1-naphthylisocyanate as an HPLC precolumn derivatization reagent for amino acid analysis is described. Derivatization is carried out by adding the isocyanate dissolved in dry acetone to a buffered amino acid solution followed by extraction of the excess reagent with cyclohexane.