✦ LIBER ✦

High-performance liquid chromatographic analysis of crystals of tRNA, aminoacyl-tRNA synthetase, and Their complex

✍ Scribed by Bernard Lorber; Richard Giegé

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 245 KB
Volume: 146
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90558-5

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✦ Synopsis

High-performance liquid chromatography on an ion exchanger column was successfully used for a rapid biochemical analysis of crystals of yeast tRNAAsp and aspartyl-tRNA synthetase as well as cocrystals formed by the synthetase and the tRNA.

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Porphyrin methyl esters and the isomers of uroporphyrin and heptacarboxylic porphyrin were separated by high-performance liquid chromatography. Isocoproporphyrin was also separated from coproporphyrin. By slight modifications to the solvent mixture, the separation of all biological polycarboxylic po