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High-performance hydroxyapatite chromatography of integral membrane proteins and water-soluble proteins in complex with sodium dodecyl sulphate

✍ Scribed by Per Lundahl; Yasushi Watanabe; Toshio Takagi


Book ID
107781743
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
828 KB
Volume
604
Category
Article
ISSN
1873-3778

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Ceramic hydroxyapatite high-performance liquid chromatography with a solvent containing high concentrations of salts was used for the separation of membrane proteins solubilized under nondenaturing and high salt conditions. The chromatographic conditions were optimized using sodium cholate as the de