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High performance density gradient electrophoresis of subcellular organelles, protein complexes and proteins

✍ Scribed by Dr. Abraham Tulp; Desirée Verwoerd; Adam Benham; Kees Jalink; Cees Sier; Jacques Neefies

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
839 KB
Volume
19
Category
Article
ISSN
0173-0835

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## High-resolution density gradient electrophoresis of proteins and subcellular organelles Following a concept developed by Bier et al. (Electrophoresis 1993, 14, [1011][1012][1013][1014][1015][1016][1017][1018], binary mixtures of amphoteric buffers with low conductivity and a good buffering capa

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## High resolution density gradient electrophoresis of cellular organelles A density gradient electrophoresis apparatus made of Perspex was constructed, with a separation column (7 X 2.2 cm) containing a 0-5% linear Ficoll gradient. The useful separation path is 6 cm. A specially designed gradient