High p21RAS expression levels correlate with chromosome 8 rearrangements in benign human mixed salivary gland tumors
✍ Scribed by Dr. Göran Stenman; Jens Sandros; Joachim Mark; Anders Nordkvist
- Book ID
- 102843381
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 807 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
The expression of RAS oncogenes in benign and malignant salivary gland tumors was studied by immunohistochemistry and by immunoblotting using monoclonal antibodies recognizing the HRASand KRASgene products. Twenty-eight out of 29 benign pleomorphic adenomas overexpressed p21 whereas only I2 out of I8 malignant salivary gland tumors expressed the p21 protein. The expression levels were also substantially higher in the adenomas than in the malignant tumors, indicating that RAS gene activation appears to be more frequent and of greater importance for benign than for malignant salivary gland tumors. Comparisons of the p21 expression levels with the karyotypes of the pleomorphic adenomas revealed a novel correlation between high p2 I expression and chromosome 8 rearrangements. As a hypothesis, it is suggested that a novel gene located on the proximal long arm of chromosome 8, most likely at band q 12, is involved in the regulation of RAS gene expression,