𝔖 Bobbio Scriptorium
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High molecular weight microtubule-associated proteins: Purification by electro-elution and amino acid compositions

✍ Scribed by K. S. Kosik; S. F. Bakalis; D. J. Selkoe; M. W. Pierce; L. K. Duffy


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
606 KB
Volume
15
Category
Article
ISSN
0360-4012

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✦ Synopsis


The high molecular weight microtubule-associated proteins (MAPs) consist of MAPs lA, lB, lC, 2A, and 2B. The proteins have been prepared from rat brain by using taxol and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The individual proteins were purified by electro-elution from stained fixed gels and shown to migrate at their original mobility without proteolysis. Amino acid compositions of purified MAP 1 and 2 component proteins are reported and compared.