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High-molecular-weight kininogen binds two molecules of cysteine proteinases with different rate constants

✍ Scribed by Boris Turk; Veronika Stoka; Vito Turk; Gunnar Johansson; Juan Jose Cazzulo; Ingemar Björk


Book ID
115932556
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
424 KB
Volume
391
Category
Article
ISSN
0014-5793

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High-affinity binding of two molecules o
✍ Boris Turk; Veronika Stoka; Ingemar Björk; Christian Boudier; Gunnar Johansson; 📂 Article 📅 1995 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 717 KB

Human low-molecular-weight kininogen (LK) was shown by fluorescence titration to bind two molecules of cathepsins L and S and papain with high affinity. By contrast, binding of a second molecule of cathepsin H was much weaker. The 2:1 binding stoichiometry was confirmed by titration monitored by los