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High-level synthesis of human prolactin in Chinese-hamster ovary cells

✍ Scribed by Carlos R. J. Soares; Ligia Morganti; Brigitte Miloux; Jan H. Lupker; Pascual Ferrara; Paolo Bartolini


Book ID
111717801
Publisher
Portland Press
Year
2000
Tongue
English
Weight
187 KB
Volume
32
Category
Article
ISSN
0885-4513

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Cycloheximide(CHM)-resistant mutant Chinese hamster ovary (CHO) and human cells were induced with N-nitrosomethylurea (NMU) and ethyl methanesulfonate (EMS); the mutants were viable and showed unlimited growth in the presence of CHM (7 X 10(-7) M), whereas this concentration inhibits protein synthes