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High-level recombinant protein production by overexpression of Mlc in Escherichia coli

✍ Scribed by Sanghee Cho; Dongwoo Shin; Geun Eog Ji; Sunggi Heu; Sangryeol Ryu

Book ID
118471302
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
285 KB
Volume
119
Category
Article
ISSN
0168-1656

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The Escherichia coli mutant CWML2 was previously reported to exhibit improved physiological characteristics, including recombinant protein production. Here we investigate the molecular basis of this phenotype by comparing the cellular level of three RNA polymerase sigma subunits by immunoblot analys