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High-Level Production of Proteolytically Sensitive Secreted Proteins in Escherichia coli Strains Impaired in the Heat-Shock Responsea

✍ Scribed by HENDRIKUS J. MEERMAN; GEORGE GEORGIOU


Book ID
119871118
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
628 KB
Volume
721
Category
Article
ISSN
0890-6564

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## Abstract High‐level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation