High-level production and isolation of human recombinant α1-proteinase inhibitor in yeast
✍ Scribed by M. Hoylaerts; A. Weyens; A. Bollen; N. Harford; T. Cabezón
- Book ID
- 115918741
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 491 KB
- Volume
- 204
- Category
- Article
- ISSN
- 0014-5793
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## Abstract Human alpha one proteinase inhibitor (α1‐PI) was cloned and expressed in __Aspergillus niger__, filamentious fungus that can grow in defined media and can perform glycosylation. Submerged culture conditions were established using starch as carbon source, 30% dissolved oxygen concentrati
There is evidence that polymorphonuclear granulocytes release neutral proteinases such as elastase (E) and cathepsin G in the course of acute leukemia. These proteinases may inactivate clotting factors by unspecific degradation before they are eliminated via complex formation with endogenous inhibit