High-level expression of the native barley α-amylase/subtilisin inhibitor in Pichia pastoris
✍ Scribed by Pernille Ollendorff Micheelsen; Peter Rahbek Østergaard; Lene Lange; Michael Skjøt
- Book ID
- 118247636
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 1002 KB
- Volume
- 133
- Category
- Article
- ISSN
- 0168-1656
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📜 SIMILAR VOLUMES
We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgate L.) seeds encoding the bifunctional ~-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence charac
Changes in bifunctional alpha-amylase/subtilisin inhibitor (BASI) expression induced by abscisic acid (ABA) were studied using in vitro cultured barley (Hordeum vulgare cv. Bonanza) embryos. The steady-state levels of BASI mRNA and BASI protein were increased by exogenously applied ABA. Accumulation
The Pichia pastoris system has the potential for very high level production of foreign proteins. This, together with the recent availability of this system in kit form, has changed Pichia from being an expression system with only specialized biotechnological applications to one that is widely used f