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High-level Expression of the Human Neurokinin-1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

✍ Scribed by Kenneth Lundström; Ann Mills; Gary Buell; Elisabeth Allet; Nadia Adami; Peter Liljeström

Book ID
115130555
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
612 KB
Volume
224
Category
Article
ISSN
1432-1327

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The cDNA for the human adenosine A 3 receptor was introduced into the pSFV1 vector, and the in vitro transcribed RNA was electroporated into baby hamster kidney (BHK) cells with pSFV-Helper RNA. This protocol resulted in packaging of a high titre Semliki Forest Virus (SFV)-A 3 virus stock. Infection