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High-level expression of foreign genes in Hansenula polymorpha

✍ Scribed by Gellissen, Gerd; Janowicz, Zbigniew A.; Weydemann, Ulrike; Melber, Karl; Strasser, Alexander W.M.; Hollenberg, Cornelis P

Book ID
122934617
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
492 KB
Volume
10
Category
Article
ISSN
0734-9750

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We developed a host-vector system for transformation and gene cloning experiments using the methylotrophic yeast Hansenula p o l y w p h a . Regeneration of protoplasts in a medium containing polyethylene glycol before plating made transformation more efficient and reproducible (2 to 3 -lo4 pg DNA).