High level expression of a heterologous protein inLactobacillus plantarumand its effect on the persistence of the recombinant strain in silage
✍ Scribed by Jane E. Rixon; Richard Sharp; Anthony G. O'Donnell; Geoffrey P. Hazlewood; John K. Brehm; Nigel P. Minton; Harry J. Gilbert
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 386 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
This paper reports the high level expression of the Staphylococcus aureus cat gene in Lactobacillus plantarum using the expression vector pMTI.500F. When the recombinant str~n of L. plantarum was grown in pure culture, CAT contributed 1.4% of the total soluble cell protein. The recombinant strain of L plantarum continued to express a high level of CAT when inoculated into silage, the heteroiogous protein constituting up to 1.75% of the total soluble cell protein. The recombinant L. plantarum strain was still able to survive and proliferate when inoculated into silage, despite its additional metabolic load.