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High-Level Expression and In Vivo Processing of Chimeric Ubiquitin Fusion Proteins in Saccharomyces Cerevisiae

✍ Scribed by Sabin, Elizabeth A. ;Lee-Ng, Chun Ting ;Shuster, Jeffrey R. ;Barr, Philip J.


Book ID
109905551
Publisher
Nature Publishing Group
Year
1989
Tongue
English
Weight
687 KB
Volume
7
Category
Article
ISSN
1087-0156

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## Abstract To develop improved reagents for mumps serology a high‐level yeast expression system was employed to produce recombinant mumps nucleoprotein (rNP). The rNP was purified by CsCl gradient centrifugation and yielded approximately 15 mg/l of yeast culture. Electron microscopy of the rNP rev