High glucose-induced growth factor resistance in human fibroblasts can be reversed by antioxidants and protein kinase C-inhibitors

✍ Scribed by Karin Hehenberger; Anders Hansson

• Book ID: 101281178
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 106 KB
• Volume: 15
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(199709)15:3<197::aid-cbf740>3.0.co;2-7

✦ Synopsis

We have studied the in¯uence of high glucose on basal ®broblast proliferation, growth factor induced cellular proliferation and the eects of antioxidants, protein kinase C-inhibitors and troglitazone. Fibroblast cultures were obtained from ®ve patients undergoing mammary reduction plastic surgery. A ¯uorometric method was used for determining total DNA in the cell samples, DNA content being proportional to cell number. D-Glucose at 15 . 5 mM and above was shown to inhibit ®broblast proliferation, and the cells were resistant to growth factors such as IGF-I and EGF at this glucose concentration. H7, bisindolylmaleimide IX, troglitazone, a-tocopherol acetate, Q10, ascorbic acid, b-carotene, DMTU and selenite were all found to reverse the high glucose-induced growth factor resistance observed in human ®broblasts. We believe that these ®ndings may be of value in the understanding and future treatment of wound healing in diabetic foot ulcers.