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High Expression of p63 Combined with a Large N/C Ratio Defines a Subset of Human Limbal Epithelial Cells: Implications on Epithelial Stem Cells

โœ Scribed by Arpitha, Parthasarathy ;Prajna, Namperumalsamy V. ;Srinivasan, Muthiah ;Muthukkaruppan, Veerappan


Book ID
115475508
Publisher
Association for Research in Vision and Ophthalmology (ARVO)
Year
2005
Weight
581 KB
Volume
46
Category
Article
ISSN
1552-5783

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A method to isolate human limbal basal c
โœ Parthasarathy Arpitha; Namperumalsamy V. Prajna; Muthiah Srinivasan; Veerappan M ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 387 KB

## Abstract The objectives were to develop method of isolating viable human limbal basal cells in order to enrich a subset of small cells with a large Nucleus/Cytoplasm (N/C) ratio expressing high levels of p63, nuclear protein. Limbal tissues were treated with trypsin for 50 min at 37ยฐC in an orbi