A method to isolate human limbal basal c
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Parthasarathy Arpitha; Namperumalsamy V. Prajna; Muthiah Srinivasan; Veerappan M
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Article
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2008
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John Wiley and Sons
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English
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## Abstract The objectives were to develop method of isolating viable human limbal basal cells in order to enrich a subset of small cells with a large Nucleus/Cytoplasm (N/C) ratio expressing high levels of p63, nuclear protein. Limbal tissues were treated with trypsin for 50 min at 37ยฐC in an orbi