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High Expression of Glycogen-Debranching Enzyme in Escherichia coli and Its Competent Purification Method

✍ Scribed by Akifumi Nakayama; Keizo Yamamoto; Shiro Tabata


Book ID
115645670
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
94 KB
Volume
19
Category
Article
ISSN
1046-5928

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Enzymes from extreme halophiles have potential as catalysts in biotransformations. We have developed methods for the expression in Escherichia coli and purification of two enzymes from Haloferax volcanii: dihydrolipoamide dehydrogenase and citrate synthase. Both enzymes were expressed in E. coli usi