High efficiency plant regeneration from petiole explants ofCarica papayaL. through organogenesis

✍ Scribed by M. Hossain; S. M. Rahman; R. Islam; O. I. Joarder

• Book ID: 104676078
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 684 KB
• Volume: 13
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00235299

✦ Synopsis

Callus cultures were obrained from petiole explants of Carica papaya on MS medium containing 0.5-10.5 μM α-naphthaleneacetic acid (NAA) in combination with 0.5-5 μM benzyladenine (BA). Hard-green calli were transferred to MS medium containing 100 mgl(-1) casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventitious buds within 2 weeks of culture. Maximum number of adventitious buds were obtained in 2 μM BA and 0.1 μM NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th week. Regenerated shoots were elongated in hormone-free medium and rooted in half-strength MS fortified with 3 UM NAA and 0.5 μM gibberellic acid (GA3). The regenerants were transferred to soil after acclimatization.