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High-Efficiency Nanoscale Liquid Chromatography Coupled On-Line with Mass Spectrometry Using Nanoelectrospray Ionization for Proteomics

✍ Scribed by Shen, Yufeng; Zhao, Rui; Berger, Scott J.; Anderson, Gordon A.; Rodriguez, Nestor; Smith, Richard D.


Book ID
127099297
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
661 KB
Volume
74
Category
Article
ISSN
0003-2700

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The coupling of column liquid chromatography on-line with mass spectrometry using post-column phosphate suppression is described. A membrane suppressor is used to remove the non-volatile phosphate ions from the mobile phase after the chromatographic separation prior to introduction into the mass spe