✦ LIBER ✦

High efficiency introduction of plasmid DNA into glycine treated Enterococcus faecalis by electroporation

✍ Scribed by Cruz-Rodz, ArmandoL. ;Gilmore, MichaelS.

Publisher: Springer
Year: 1990
Tongue: English
Weight: 354 KB
Volume: 224
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00259462

No coin nor oath required. For personal study only.

✦ Synopsis

A highly efficient electroporation system for Enterococcus faecalis was developed by systematically optimizing different parameters. One parameter found to be particularly critical for electroporation was cultivation of E. faecalis in medium containing a high glycine concentration, prior to electroporation. Osmotic stabilization of cells with 0.5 M sucrose was also found to be critical during glycine treatment. 10(6) transformants per microgram of plasmid DNA were consistently obtained within 48 h. Electrocompetent preparations of E.-faecalis could be stored at - 70 degrees C without loss of competence.

📜 SIMILAR VOLUMES

Efficient gene delivery into murine ovar

Efficient gene delivery into murine ovarian cells by intraovarian injection of plasmid DNA and subsequent in vivo electroporation

✍ Masahiro Sato; Maya Tanigawa; Natsuko Kikuchi; Shingo Nakamura; Minoru Kimura 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 396 KB

## Abstract Summary: We describe the use of direct injection of circular plasmid DNA and subsequent in vivo electroporation (EP) for efficient gene delivery to the ovarian cells, including follicular cells and oocytes of mice. When Trypan blue (TB) was injected into the central portion of an ovary