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High efficiency HPLC with long packed columns

✍ Scribed by Dewaele, C. ;Verzele, M.

Book ID
102343429
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
236 KB
Volume
3
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Coupling several 25 cm Γ— 2 mm ID analytical columns together is a simple and easy way to achieve high resolution HPLC (20,000–50,000 plates). How to do this with commercially available instruments, injectors and detectors is described in the paper.

An attractive feature of the long narrow column approach is that the same HPLC set‐up can be used for both fast‐low and slow‐high efficiency separations.

High efficiency is essential for really complex natural mixtures, e.g. the hop and beer bitter acids. Some examples are shown.

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Use of high-efficiency packed columns fo
Use of high-efficiency packed columns for gas-solid chromatography : V. Micropacked columns
✍ Fabrizio Bruner; Paolo Ciccioli; Giuliano Bertoni πŸ“‚ Article πŸ“… 1974 πŸ› Elsevier Science 🌐 English βš– 526 KB

## Micropacked columns are constructed using graphitized carbon black as a support for gas-liquid-solid chromatography. Their effectiveness is evaluated in terms of Van Deemter plots, flow-rates, inlet pressure and column capacity. It is shown that such columns, by choosing an appropriate liquid