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High Efficiency Genetic Transformation in Maize Induced by Exogenous DNA

✍ Scribed by Korohoda, J.; StrzaŁka, K.


Book ID
122289543
Publisher
Elsevier
Year
1979
Weight
682 KB
Volume
94
Category
Article
ISSN
0044-328X

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Efficient regeneration (80%) and high frequency genetic transformation (10-33%) were achieved by culturing protoplasts isolated from hypocotyl tissues of six day old Brassica oleracea seedlings and by subjecting these protoplasts to PEG mediated direct plasmid uptake. Three different plasmid vectors