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High-density oligonucleotide arrays

✍ Scribed by A.P. Blanchard; R.J. Kaiser; L.E. Hood

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
345 KB
Volume
11
Category
Article
ISSN
0956-5663

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✦ Synopsis

We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniamri~ conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a chemically modified silicon dioxide surface, where they react to synthesize DNA. The drops are confined to small areas by a pattern of hydrophobic surface modifications, thus allowing the formation of high-density arrays without mixing between adjacent drops.

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