High density culture of hybridoma cells
High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge
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Michiyuki Tokashiki; Takami Arai; Kimihiko Hamamoto; Kenji Ishimaru
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Article
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1990
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Springer
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English
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The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation a