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High-copy-number derivatives of the plasmid cloning vector pBR322

✍ Scribed by Boros, Imre; Pósfai, György; Venetianer, Pál


Book ID
125473864
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
306 KB
Volume
30
Category
Article
ISSN
0378-1119

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Mutations in a new chromosomal gene of E
✍ Lopilato, Jane ;Bortner, Scott ;Beckwith, Jon 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 711 KB

We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 rain on the E. coli chromosome. This locus is distinct from