✦ LIBER ✦

High Content Image Cytometry : A Versatile Tool for Mapping Subnuclear Organization

✍ Scribed by Winnok H. De Vos; P. Van Oostveldt; E. M. M. Manders

Publisher: Wiley (John Wiley & Sons)
Year: 2009
Weight: 855 KB
Volume: 11
Category: Article
ISSN: 1439-4243
DOI: 10.1002/imic.200990089

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✦ Synopsis

The human eye is a powerful and sensitive instrument which excels in pattern recognition, by using mental templates to identify and categorize objects. Unfortunately, its exact strength may also be its weakness, for it implies a certain subjectivity to the interpretation of images. Furthermore, our distinctive power and vocabulary for describing objects in a quantitative manner are limited. Accurate visualization and characterization of cellular features thus calls for a more objective approach, which can now be provided by the fast growing discipline of high content image cytometry (HCIC). logical assays, focused on the biology of the cell nucleus.

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## Abstract The organization of proteins in space and time is essential to their function. To accurately quantify subcellular protein characteristics in a population of cells with regard for the stochasticity of events in a natural context, there is a fast‐growing need for image‐based cytometry. Si