High cell kinetics is associated with amplification of the int-2, bcl-1, myc and erbB-2 proto-oncogenes and loss of heterozygosity at the DF3 locus in primary breast cancers
✍ Scribed by Alma Contegiacomo; Claudia Pizzi; Laura De Marchis; Maurizio Alimandi; Paolo Delrio; Ester Di Palma; Giuseppe Petrella; Laura Ottini; Deborah French; Luigi Frati; Angelo R. Bianco; Renato Mariani-Costantini
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- French
- Weight
- 773 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Cell kinetics, is a predictive parameter of breast-cancer aggressiveness, and mutations occurring in mammary tumorigenesis may favor iuncontrolled cell proliferation. In this study, cell kinetics, clinico-pathological characteristics and genetic alterations at the irrt-2, bcl-I, c-myc, c-erbB-2, and DF3 loci were analyzed and correlated in 54 primary breast carcinomas. The occurrence of mutations at more than one locus was also studied. Tumor-proliferative activity was evaluated by determination of the thymidine labeling index (TLI). Amplification (AMP) of int-2 was observed in I I .2%, of bcl-I in 9.4%, of c-myc in 5.7% and of c-erbB-2 in 8.6% of the carcinomas. Loss of heterozygosity (LOH) at the DF3 locus was detected in 13.9% of the tumors. Genetic alterations demonstrated a significant association with patient's age and high TLI values. AMP and LOH + AMP did not appear to be statistically related to histotype. histological grade, tumor size or lymph-node status. Alone, allele loss at the DF-3 locus was not significantly associated with any of the clinico-pathological characteristics studied. Alterations at more than one locus, including int-21bcl-I, int-2/ c-myc, int-21bcl-I lc-erbB-2, and c-mycIDF3, were detected in