𝔖 Bobbio Scriptorium
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High affinity concanavalin a binding to sterol-depleted L cells

✍ Scribed by Jan D. Marshall; Hans-Jörg Heiniger; Charity Waymouth

Book ID
102883289
Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
881 KB
Volume
100
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Binding of Concanavalin A to mouse L cells which were grown in serum free, chemically defined medium and depleted of their membrane sterol by blocking their de novo sterol synthesis was investigated. Kinetic analysis of binding data implied positive cooperativity, with two different affinities for Con A, in both experimental and control cultures. The amount of Con A bound to the cell surface at saturation was approximately 0.5 picomoles per mg cellular protein in controls and approximately 1.0 picomoles per mg cellular protein in 25‐hydroxycholesterol treated cultures (which had a reduced sterol concentration of up to 50% in their plasma membranes). This phenomenon was reversed when cholesterol or mevalonate was added to the inhibited cultures to compensate for their inability to synthesize sterol. Our findings indicate that lectin binding to specific glycoprotein receptors is influenced by membrane lipid composition.

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Low- and high-affinity concanavalin A binding to thymocyte plasma membrane vesicles
✍ Donald F. H. Wallach; Rupert Schmidt-Ullrich 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 257 KB

## Abstract Binding of concanavalin A to isolated thymocyte membrane vesicles occurs through (a) numerous (∼6 × 10^6^/cell equivalent) low‐affinity sites (K~a~ = 1.3 × 10^5^ M^−1^) and (b) fewer (∼0.4 × 10^6^/cell equipment) specific receptors (K~a~ = 6.8 × 10^6^ M^−1^) defined as 55,000 D glycopro