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High-Affinity Calcium-Binding Site in the γ-Carboxyglutamic Acid Domain of Bovine Factor VII †

✍ Scribed by Inoue, Keisuke; Shimada, Hidenori; Ueba, Junichi; Enomoto, Satoru; Tanaka-Saisaka, Yukari; Kubota, Takahiro; Koyama, Masayoshi; Morita, Takashi


Book ID
121862259
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
352 KB
Volume
35
Category
Article
ISSN
0006-2960

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High and low affinity binding sites for
✍ David Moscatelli 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 834 KB

Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind