𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Heterotopic limb allotransplantation model to study skin rejection in the rat

✍ Scribed by Jamal A. Nazzal; Timothy S. Johnson; Chad R. Gordon; Mark A. Randolph; W.P. Andrew Lee

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
415 KB
Volume
24
Category
Article
ISSN
0738-1085

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Current rodent models for investigation of limb allotransplantation typically utilize orthotopic whole‐limb transplantation, a morbid and time‐consuming procedure. Our objective was to design a less morbid rat model to explore the immunological obstacles of limb transplantation, and particularly skin. Twenty lower hindlimbs from 10 donors were transplanted into a heterotopic subcutaneous position into 20 animals (10 isogeneic and 10 allogeneic). Each group was further subdivided to include animals with (n = 5) and without (n = 5) a skin paddle for observation of cutaneous signs of rejection. All grafts in the isogeneic group survived for 100 days, i.e., the endpoint of the study. Allogeneic transplants rejected their allografts at a mean of 12.8 days (with skin) and 20.6 days (without). Our heterotopic limb transplantation model takes less time and is less stressful to the animals, while allowing for early observation of graft skin rejection, when compared to orthotopic whole‐limb transplantation. © 2004 Wiley‐Liss, Inc. Microsurgery 24:448–453, 2004.

📜 SIMILAR VOLUMES

A new rat model to study the correlation
A new rat model to study the correlation of cardiac and skeletal muscle allograft rejection
✍ Jacqueline Blanchard; Malek Massad; Marin Sekosan; Ty Dunn; Vandad Raofi; Dawn H 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB 👁 2 views

As a first step to study the correlation of cardiac and skeletal muscle allograft rejection, we describe a new experimental rat model of simultaneous heterotopic heart and cutaneous maximus muscle flap allotransplant. Brown Norway rats were used as donors and Lewis rats as recipients. No immunosuppr

T1 relaxation times for viability evalua
T1 relaxation times for viability evaluation of the engrafted and the native liver in a rat model of heterotopic auxiliary liver transplantation: a pilot study
✍ Ye-Dong Fan; Bart Vanzieleghem; Eric Achten; Yves De Deene; Luc Defreyne; Marlee 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 488 KB

Following a heterotopic auxiliary liver transplantation, commonly used measurements are either invasive or non-indicative of individual viability of the coexisting engrafted and native livers. Magnetic resonance imaging (MRI) was therefore tested for its potential to monitor the post-transplant hepa

Selection of the sacral nerve posterior
Selection of the sacral nerve posterior roots to establish skin–CNS–bladder reflex pathway: An experimental study in rats
✍ Jinwu Wang; Chunlin Hou; Jia Jiang; Qi Li; Feng Zhang 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 335 KB

## Abstract The purpose of this study was to explore the innervations of different sacral nerve posterior roots to bladder, and to provide evidence for further study of skin–CNS–bladder reflex pathway in the spinal cord injury patient. Spinal cord injury was produced in 10 rats. The bilateral spina

Cultures of rat astrocytes challenged wi
Cultures of rat astrocytes challenged with a steady supply of glutamate: New model to study flux distribution in the glutamate–glutamine cycle
✍ Luís L. Fonseca; Miguel A.R. Monteiro; Paula M. Alves; Manuel J.T. Carrondo; Hel 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 390 KB 👁 1 views

## Abstract Glutamate metabolism in astrocytes was studied using an experimental setup that simulates the role of neurons (glutamate producers and glutamine consumers) by the addition of glutaminase to the culture medium. Thereby, a steady supply of glutamate was imposed at the expense of glutamine

JTE-522, a selective COX-2 inhibitor, in
JTE-522, a selective COX-2 inhibitor, interferes with the growth of lung metastases from colorectal cancer in rats due to inhibition of neovascularization: A vascular cast model study
✍ Hirotoshi Kobayashi; Tsuyoshi Gonda; Hiroyuki Uetake; Tetsuro Higuchi; Masayuki 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 French ⚖ 506 KB

## Abstract The lung is a frequent site of metastasis from colorectal cancer, but angiogenesis of lung metastases has not been clarified. Some COX‐2 inhibitors prevent tumor growth, although the inhibitory mechanism at the metastatic site is obscure. We investigated the microvascular structure of s