Heterologous Protein Production in CHO Cells: Methods and Protocols

✍ Scribed by Paula Meleady (eds.)

Publisher: Humana Press
Year: 2017
Tongue: English
Leaves: 256
Series: Methods in Molecular Biology 1603
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.

✦ Table of Contents

Front Matter....Pages i-x
Back Matter....Pages 1-23
....Pages 25-44

✦ Subjects

Protein Science

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