Heterologous production insaccharomyces cerevisiae of differentaspergillus nidulans xylanases of potential interest in oenology

✍ Scribed by Ganga, Angélica; Querol, Amparo; Vallés, Salvador; Ramón, Daniel; MacCabe, Andrew; Piñaga, Francisco

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 237 KB
• Volume: 78
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199811)78:3<315::aid-jsfa118>3.0.co;2-1

✦ Synopsis

Three Aspergillus nidulans endo-b-(1,4)-xylanases and (X 22 , X 24 X 34 ) have been produced in Saccharomyces cerevisiae laboratory strains. In fermenter cultivation, the resulting strains were able to secrete the heterologous xylanases when grown in minimal medium containing glucose as carbon source, yielding production levels between 0É5 and 1É0 U ml~1. From such a culture, the xylanases were puriÐed in a single chromatographic step and then physico-chemically characterised. The yeast derived enzymes had the same enzymatic and biochemical properties as the fungal xylanases (ie molecular weights, pI values, pH and temperature optima, Michaelis constants and pH and temperature K m stabilities). The heterologously produced enzymes retained maximum activity in the presence of 10% (w/v) glucose or 10% (w/v) fructose, concentrations normally found in musts. However, ethanol at 12% (v/v) caused inhibitions of 20% for and and 48% for 1998 Society of Chemical Industry. X 22 X 24 X 34 .