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Heterologous gene expression in the basidiomycete fungusCoprinus cinereus

✍ Scribed by Lorna A. Casselton; Alicia Fuente Herce

Publisher
Springer-Verlag
Year
1989
Tongue
English
Weight
555 KB
Volume
16
Category
Article
ISSN
0172-8083

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✦ Synopsis

Direct selection for TRP + transformants of a trp-2 mutant of Coprinus cinereus showed that the gene mutation could be complemented by the heterologous gene from two other basidiomycete species, Schizophyllum commune (trpl) and Phanerochaete ehrysosporium (trpC) but not by an ascomycete gene from Aspergillus nidulans (trpC). Cotransformation was used to confirm that the Aspergillus nidulans gene could be integrated but not expressed. Cotransformations were also used to show that the Aspergillus nidulans isocitrate lyase gene (acuD) and a construct containing the bacterial HygB gene fused to a hemibasidiomycete gene promotor from Ustilago maydis were also unexpressed in Coprinus cinereus.

πŸ“œ SIMILAR VOLUMES

Transformation as a method of increasing
Transformation as a method of increasing gene copy number and gene expression in the basidiomycete fungusCoprinus cinereuss
✍ Fiona M. Mellon; Lorna A. Casselton πŸ“‚ Article πŸ“… 1988 πŸ› Springer-Verlag 🌐 English βš– 567 KB

Seven transformants having varying numbers of non-homologously integrated copies of the isocitrate lyase gene, acu-7, were analysed for enzyme activity. Maximum levels of activity, 3.8 times that of the wild type, were observed in a transformant with only two gene copies whereas eight gene copies in