Heterologous expression of alcohol oxidase in Saccharomyces cerevisiae: properties of the enzyme and implications for microbody development

Directed evolution of a secretory leader

Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae

✍ J. Andy Rakestraw; Stephen L. Sazinsky; Andrea Piatesi; Eugene Antipov; K. Dane 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 474 KB 👁 2 views

## Abstract Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in __Saccharomyces__ __cerevisiae__. These strategies typically involve altering the expression of a native protein thought