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Heterologous expression of a gene encoding a thermostable β-galactosidase fromAlicyclobacillus acidocaldarius

✍ Scribed by Tiezheng Yuan; Peilong Yang; Yaru Wang; Kun Meng; Huiying Luo; Wei Zhang; Ningfeng Wu; Yunliu Fan; Bin Yao

Publisher
Springer Netherlands
Year
2007
Tongue
English
Weight
236 KB
Volume
30
Category
Article
ISSN
0141-5492

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✍ Maria J. Torres; Byong H. Lee 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 415 KB

A B-galactosidase gene from Lactobacillus sake coding for lactose hydrolysis was cloned and expressed in Escherichia coli. Chromosomal DNA from L. 'sa& was partially digested with the restriction enzyme SaDAI, and the 3-6 Kb fragment was ligated to the cloning vector pSP72 digested with BarnHI. One