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Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme

✍ Scribed by Fabiana M. de Almeida; Beatriz M. Bonini; Daniela Beton; João A. Jorge; Héctor F. Terenzi; Aline M. da Silva


Book ID
116828931
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
207 KB
Volume
65
Category
Article
ISSN
1046-5928

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In Neurospora crassa the qa-2 gene, which encodes catabolic dehydroquinase, is under positive control exerted by the inducer quinic acid and an activator protein encoded in the closely linked qa-1 gene. In order to determine if this regulatory mechanism is maintained when the qa-2 gene is cloned on