We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell
Heterologous expression in Bacillus subtilis II. In vitro removal of the attenuator sequence of the Escherichia coli his operon allows expression of the cloned hisG gene in B. subtilis
โ Scribed by Buvoli, M.; Damiani, G.; Ferretti, L.; Testori, A.; Sgaramella, V.
- Book ID
- 122866945
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 954 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0378-1119
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## Abstract A novel metabolic engineering technique involving the redirection ofcellular carbon fluxes was employed to reduce acetate production in an __Escherichia coli__ culture. Metabolic engineering was achieved by cloning __E. coli__ the gene for the __Bacillus subtilis__ acetolactate synthase