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Heterogeneous flexibilities of the active site domains of homodimeric creatine kinase: effect of substrate

โœ Scribed by Steven H. Grossman; Richard M. France; James R. Mattheis


Book ID
113268725
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
682 KB
Volume
1159
Category
Article
ISSN
0167-4838

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Six fully conserved arginine residues (R129, R131, R235, R291, R319, and R340) closely grouped in the nucleotide binding site of rabbit muscle creatine kinase (rmCK) were mutated; four to alanine and all six to lysine. Kinetic analyses in the direction of phosphocreatine formation showed that all fo