✦ LIBER ✦
Heterogeneity of lysosomal enzymes in cultured normal and sialidosis type II human fibroblasts and the effect of ammonium chloride on this heterogeneity
✍ Scribed by Clare M. Heyworth; Colin H. Wynn
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 433 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0300-8177
No coin nor oath required. For personal study only.
✦ Synopsis
Lysosomal enzymes of human skin fibroblasts sediment in a Percoll gradient with major peaks at 1.05 and 1.10 g cm-3. The distribution of enzyme activity between the two peaks is dependent on growth state of the cells. Sialidosis Type II fibroblasts show a normal distribution pattern. NH4Cl stimulates enzyme secretion and de novo enzyme synthesis.