Heterogeneity of Human Serum Albumin
β Scribed by HOCH, H.; MORRIS, C. J. O. R.
- Book ID
- 109546623
- Publisher
- Nature Publishing Group
- Year
- 1945
- Tongue
- English
- Weight
- 137 KB
- Volume
- 156
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/156234a0
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π SIMILAR VOLUMES
Human serum albumin (HSA) is used in large amounts as an excipient in many biopharmaceutical formulation to prevent loss of the active ingredient through adsorption and/or degradation. Traditionally, iso-electric focusing has been used to demonstrate charge heterogeneity in HSA preparations. In an e
Binding equilibria for decanoate, octanoate, and hexanoate to defatted human serum albumin were investigated by dialysis exchange rate determinations in 66 mM sodium phosphate buffer, pH 7.4, 37 degrees C. The binding isotherms for decanoate and octanoate could not be fitted by the general binding e