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Heterogeneity of auxin-accumulating membrane vesicles fromCucurbitaandZea: a possible reflection of cell polarity

✍ Scribed by Martin Lützelschwab; Han Asard; Ulrike Ingold; Rainer Hertel

Book ID
104759454
Publisher
Springer-Verlag
Year
1989
Tongue
English
Weight
824 KB
Volume
177
Category
Article
ISSN
0032-0935

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✦ Synopsis

When microsomes from hypocotyls of Cucurbita pepo L. or coleoptiles of Zea mays L. were centrifuged on dextran-sucrose gradients a heterogeneity of auxin-accumulating vesicles was observed. Vesicles from the top part of the gradient showed saturable, specific accumulation of indole-3-acetic acid with only a small stimulation by phytotropins, and with very few binding sites for 1-N-naphthylphthalamic acid. In the vesicles from the lower part of the gradient, net accumulation of indole-3-acetic acid could be strongly increased by addition of phytotropins; binding of 1-N-naphthylphthalamic acid was high in this region. After two-phase partitioning, both kinds of vesicles were found in the upper-phase membrane fraction considered to be purified plasma membrane. The hypothesis is discussed that vesicles can be separated from the apical and basal parts of the cell's plasmalemma.

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