𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Heterogeneity in the conformation of different protein fractions from the human erythrocyte membrane

✍ Scribed by Holzwarth, G. ;Yu, John ;Steck, Theodore L.

Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
437 KB
Volume
4
Category
Article
ISSN
0091-7419

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We have isolated 5 families of proteins from human red blood cell membranes and characterized their secondary structure by ultraviolet circular dichroism measurements. The protein families were prepared by selective solubilization from ghosts under nondenaturing conditions. We find that the intact ghost has a mean α‐helix fraction of 0.37, whereas a low‐ionic‐strength extract (bands 1, 2, 5, β€œspectrin”) has a substantially higher helix fraction, 0.55. Further extraction of the ghosts with para‐chloromercuribenzoate yields bands 2.1, 4.1, 4.2, and 6; their helix content is only 0.17. Finally, the major intrinsic protein, band 3, was solubilized by a nonionic detergent. Its helix fraction is 0.38.

πŸ“œ SIMILAR VOLUMES

Substrate heterogeneity of component a o
Substrate heterogeneity of component a of the human erythrocyte membrane
✍ Roses, Allen D. πŸ“‚ Article πŸ“… 1976 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 323 KB

## Abstract Component a of the erythrocyte membrane is a specific substrate for endogenous protein kinase activity and its phosphorylation is significantly decreased under assay conditions in myotonic muscular dystrophy (Roses, A. D., and Appel, S. H., J. Membr. Biol. 20:51–58 (1975)). We have demo

Transport of drugs through human erythro
Transport of drugs through human erythrocyte membranes: pH dependence of drug transport through labeled human erythrocytes in the presence of band 3 protein inhibitor
✍ Yasuhiro Matsumoto; Masahiko Ohsako πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 396 KB

To reveal the role of the band 3 anion transport protein of the erythrocyte membrane in drug transport through the membrane, the possible effects of inhibitors of anion transport on the permeability of some anionic drugs were examined. The amounts of these drugs that permeated varied markedly with t