We have prepared several truncated versions of the Choristoneura fumiferana ecdysone receptor (CfEcR) cDNA to identify domains required for heterodimerization with C. fumiferana ultraspiracle (CfUSP). The CfEcR protein containing all six domains bound to hsp27 EcRE in the presence of CfUSP protein.
โฆ LIBER โฆ
Heterodimerization of ecdysone receptor and ultraspiracle on symmetric and asymmetric response elements
โ Scribed by Srini C. Perera; Sichun Zheng; Qi-Li Feng; Peter J. Krell; Arthur Retnakaran; Subba R. Palli
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 415 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0739-4462
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## Abstract Genomic recombination was performed in a genetically modified stable HeLa cell line, HeLa55, using a uniquely designed donor vector harboring an exchange cassette comprised of the human glucocorticoid receptor (hGR) gene, its response element, and a luciferase reporter gene, to generate