Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro

✍ Scribed by Shin Shimaoka; Ryoji Tsuboi; Toshimasa Jindo; Ryusuke Imai; Kenji Takamori; Jeffrey S. Rubin; Hideoki Ogawa

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 971 KB
• Volume: 165
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041650214

✦ Synopsis

Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional polypeptide which acts as mitogen, motogen, or morphogen. In this study, we examined the effect of HGF/SF on human hair growth using organ and cell culture systems. HGF/SF was found to stimulate hair length and DNA synthesis in hair follicles at increasing concentrations up to 10 ng/ml ( P < 0.05 and P < 0.01, respectively). HGF/SF stimulated ['Hlthymidine incorporation by hair bulb-derived keratinocytes with the strongest response at 30 ng/ml of HGF/SF ( P < 0.05). Cultured follicular papilla cells secreted HGF/SF, measured by an enzyme-linked immunoassay, in response to interleukin 1 -a (IL1 -a, 10 ng/ml), tumor necrosis factor-a (TNF-a, 10 ng/ml), or tetradecanoylphorbolacetate (1 00 nM) at levels ranging from 0.2 to 0.3 ndmg protein/48 h. HGF/SF mRNA expressions, measured by the reverse transcription-polymerase chain reaction, were detected in follicular papilla cells, and were also stimulated by the three reagents. Transforming growth factor-p (1 0 ng/ml) suppressed both protein and mRNA levels. These results suggest that hair follicle elongation induced by HGF/SF in organ culture occurs partly due to the mitogenic activity of HGF/SF expressed in follicular papilla cells on hair bulb-derived keratinocytes. o 1995 WtIey-Liss, Inc.