Hepatocyte growth factor (HGF) produced by peritoneal fibroblasts may affect mesothelial cell morphology and promote peritoneal dissemination
✍ Scribed by Masakazu Yashiro; Yong-Suk Chung; Tohru Inoue; Shigehiko Nishimura; Tasuku Matsuoka; Tomofumi Fujihara; Michio Sowa
- Book ID
- 102652316
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 518 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Mesothelial cell monolayers have been reported to infiltration of cancer cells into the peritmum. Gt previously reported that peritoneal fibrosis induced by gastric cancer cells prior to metastatization may provide a congenial environment for peritoneal mtastases. In this study, we investigatedtheeffectsofperitonealfibroblastsonperitoncalmesotheliil cell morphology. Human gastric cancer (OCUM-ZMD3). peritoneal fibroblast (NF-ZP) and mesothelial (MS-I) cell lines ware established in our laboratory. Histology of the peritoneum was investigated following intrap*itoncal inoculation of serumfree conditioned media (SF-CM) from OCUM-ZMD3 celk into nude mice. SF-CM horn peritoneal fibroblasts was added to mondayer-cultured merothelial celk, and their morphology was examined by phase-contrast microscopy. This experiment was conducted in the presence and absemce of neutralizing antibodies against various factors. Mesothelial celk exposed to fibroblasts proliferation became hemispherical and separated from each other, while unexpOScd mesothelium mmained as a flat monolayer. Cultured-memthelial cells rounded up or urhibited a fibroblast-like shape following the addition of fibroblast SF-CM. Anti-hepatocyte growth factor ( H G g : z king antibody partly inhibited this effect. We suggest that soluble factors, such as HGF, produced by perkond fibroblasts affect the morphology of mcsothelial cells in monolayers so that the resulting environment may become prone to the peritoneal dimmination of cancer celk.