Hepatocellular adenoma subtype classification using molecular markers and immunohistochemistry

✍ Scribed by Paulette Bioulac-Sage; Sandra Rebouissou; Cristel Thomas; Jean-Frédéric Blanc; Jean Saric; Antonio Sa Cunha; Anne Rullier; Gaëlle Cubel; Gabrielle Couchy; Sandrine Imbeaud; Charles Balabaud; Jessica Zucman-Rossi

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 458 KB
• Volume: 46
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.21743

✦ Synopsis

Hepatocellular adenomas (HCA) with activated beta-catenin present a high risk of malignant transformation. To permit robust routine diagnosis to allow for HCA subtype classification, we searched new useful markers. We analyzed the expression of candidate genes by quantitative reverse transcription polymerase chain reaction QRT-PCR followed by immunohistochemistry to validate their specificity and sensitivity according to hepatocyte nuclear factor 1 alpha (HNF1alpha) and beta-catenin mutations as well as inflammatory phenotype. Quantitative RT-PCR showed that FABP1 (liver fatty acid binding protein) and UGT2B7 were downregulated in HNF1alpha-inactivated HCA (P

Conclusion:

We refined hca classification and its phenotypic correlations, providing a routine test to classify hepatocellular adenomas using simple and robust immunohistochemistry.