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Hepatitis B virus replication and viral antigen synthesis in hepatocyte lines derived from normal human liver

โœ Scribed by Jasper zu Putlitz; Eve A Roberts; Stefan Wieland; Yumi Kono; Hubert E Blum

Book ID
117366434
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
129 KB
Volume
52
Category
Article
ISSN
0168-1702

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An efficient method for cloning subgenomic fragments of the hepatitis B virus (HBV) was developed, utilizing its abundant single-stranded replicative intermediates. The total genomic DNA obtained from the liver tissue of patients with chronic HBV infection was treated by using the Klenow fragment of