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Hepatitis A Synthetic Peptide VP3(110–121) Miscibility with Dipalmitoylphosphatidylcholine, Dipalmitoylphosphatidylglycerol, and Stearylamine Monolayers

✍ Scribed by P. Sospedra; M.A. Alsina; M. Espina; F. Reig; I. Haro; C. Mestres

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
82 KB
Volume
221
Category
Article
ISSN
0021-9797

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✦ Synopsis

To prepare liposomes containing a synthetic hepatitis A virus antigen (HAV) [VP3(110-121)] as a vaccine, the miscibility of this peptide (with negative net charge) with a neutral lipid [dipalmitoylphosphatidylcholine (DPPC)], a negatively charged lipid [dipalmitoylphosphatidylglycerol (DPPG)], and a positively charged lipid [Stearylamine (SA)] was studied through compression isotherms of monolayers. Mixtures with DPPC and SA showed a low degree of interaction with the peptide, the composition of the monolayer being stable through compression. For DPPGcontaining monolayers larger positive deviations from ideality were found, and the peptide was squeezed out from the monolayer at a DPPG/VP3(110-121) mole fraction of 0.8/0.2. All this suggests that besides hydrophobic interactions between the peptide and the lipid, electrostatic forces also play a role; thus it seems that neutral and positively charged lipids would be more suitable for preparing stable liposomes with VP3(110-121).

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Synthetic peptide vaccines are safer and cheaper than classical ones, but immunoresponse is usually lower. The early interaction of hepatitis A virus (HAV) with cells during the infection and immunological response processes is not as well-known as for other picornavirus. However, it seems that elec